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The Microscope and Discovery of Microorganisms. Antonie van Leeuwenhoek (1632–1723) was one of the first people to observe microorganisms, using a microscope of his own design, and made one of the most important contributions to biology. Robert Hooke was the first to use a microscope to observe living things
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Leeuwenhoek made microscopes consisting of a single high-quality lens of very short focal length; at the time, such simple microscopes were preferable to the compound microscope, which increased the problem of chromatic aberration. Although Leeuwenhoek’s studies lacked the organization of formal scientific research, his powers of careful observation enabled him to make discoveries of fundamental importance. In 1674 he likely observed protozoa for the first time and several years later bacteria. Those “very little animalcules” he was able to isolate from different sources, such as rainwater, pond and well water, and the human mouth and intestine. He also calculated their sizes.