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Answer 2

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Starch testing

Iodine solution is used to test leaves for the presence of starch. You need to:

heat a plant leaf in boiling water for 30 seconds (this stops its chemical reactions)

heat it in boiling ethanol for a few minutes (this removes most of its colour)

wash with water and spread onto a white tile

add iodine solution from a dropping pipette

After a few minutes, the parts of the leaf that contain starch turn blue-black.

Note that ethanol is heated using a hot water bath. Ethanol boils at 78°C, so a tube of it boils when placed in a beaker of hot water. This is safer than using a Bunsen burner because ethanol is flammable.