plzzz help
tell the conditions that effect hybridisation!!!!
Answers
Factors Affecting Hybridization
Because hybridization involves nucleotide bases and the separation and joining or reannealing of strands, several environmental factors can influence this process. Examples of environmental factors that may affect hybridization include:
Temperature: If the temperature is too high, the strands melt. If it is too low, they might be forced together.
The pH: A pH that is too alkaline will cause the strands to separate; too acidic and they are forced together.
The guanine to cytosine ratio (G:C ratio): Since this bond is stronger than the other nucleotide bonds, if the G:C ratio in the desired target strand is high, the separation process may take longer or require higher energy (temperature).
Salt concentration in the hybridization buffer.