postulates of Robert koch
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1.The microorganism must be found in abundance in all organisms suffering from the disease, but should not be found in healthy organisms.
2.The microorganism must be isolated from a diseased organism and grown in pure culture.
3.The cultured microorganism should cause disease when introduced into a healthy organism.
4.The microorganism must be reisolated from the inoculated, diseased experimental host and identified as being identical to the original specific causative agent.
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