Question

Predict the most likely observed level of luciferase activity if plasmid pCDγ3-789 is introduced into nonlymphoid cells such as a line of kidney tissue cells. Provide reasoning to justify your prediction.

Answer 1

if the plasmid is introduced into the non lymphoid cells such as a line of kidney tissue there will be incorporation of the nuclear material into the DNA present in the kidney tissue.

Explanation:

• The plasmid is actually e a round shape paid extra nuclear ring of chromosomal DNA which is present mostly in the bacteria. However if they are placed in contact with the human genome they have the capacity to incorporate themselves.
• As a result of this there will be error in the transcription and translation and hence the protein synthesis will differ resulting in various number of ill effects in the kidney cell

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