Question

preparation of blood agar​

Answer 1

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Prepare the blood agar base as instructed by the manufacturer. Sterilize by autoclaving at 121°C for 15 minutes.

Transfer thus prepared blood agar base to a 50°C water bath.

When the agar base is cooled to 50°C, add sterile blood agar aseptically and mix well gently. Avoid formation of air bubbles.  You must have warmed the blood to room temperature at the time of dispensing to molten agar base.

(Note:  If you are planning to prepare a batch of blood agar plates, prepare few blood agar plates first to ensure that blood is sterile).

Dispense 15 ml amounts to sterile petri plates aseptically

Label the medium with the date of preparation and give it a batch number (if necessary).

Store the plates at 2-8°C, preferably in sealed plastic bags to prevent loss of moisture.  The shelf life of thus prepared blood agar is up to four weeks.



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