Preparation of stained temporary mount of onion peel and human chick cell
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Onion peel:-
Pour some distilled water into a watch glass.
Peel off a leaf from half a piece of onion and using the forceps, pull out a piece of transparent onion peel (epidermis) from the leaf.
Put the epidermis in the watch glass containing distilled water.
Take a few drops of safranin solution in a dropper and transfer this into another watch glass.
Using a brush, transfer the peel into the watch glass containing the safranin solution.
Let this remain in the Safranin solution for 30 seconds, so that the peel is stained.
Take the peel from the Safranin solution using the brush and place it in the watch glass containing the distilled water.
Take a few drops of glycerine in a dropper and pour 2-3 drops at the center of a dry glass slide.
Using the brush, place the peel onto the slide containing glycerine.
Take a cover slip and place it gently on the peel with the aid of a needle.
Remove the extra glycerine using a piece of blotting paper.
Place this glass side on the stage of the compound microscope and view it.
Cheek cell:-
Gently scrape the inner side of the cheek using a toothpick, which will collect some cheek cells.
Place the cells on a glass slide that has water on it.
Mix the water and the cheek cells using a needle and spread them.
Take a few drops of Methylene blue solution using a dropper and add this to the mixture on the slide.
After 2-3 minutes remove any excess water and stain from the slide using a blotting paper.
Take a few drops of glycerine using a dropper and add this to the test mixture.
Take a clean cover slip and lower it carefully on the mixture with the aid of a needle.
Using a brush and needle, press the cover slip gently to spread the epithelial cells.
Remove any extra liquid around the cover slip using a blotting paper.
Place this glass side on the stage of the compound microscope and view it.
Pour some distilled water into a watch glass.
Peel off a leaf from half a piece of onion and using the forceps, pull out a piece of transparent onion peel (epidermis) from the leaf.
Put the epidermis in the watch glass containing distilled water.
Take a few drops of safranin solution in a dropper and transfer this into another watch glass.
Using a brush, transfer the peel into the watch glass containing the safranin solution.
Let this remain in the Safranin solution for 30 seconds, so that the peel is stained.
Take the peel from the Safranin solution using the brush and place it in the watch glass containing the distilled water.
Take a few drops of glycerine in a dropper and pour 2-3 drops at the center of a dry glass slide.
Using the brush, place the peel onto the slide containing glycerine.
Take a cover slip and place it gently on the peel with the aid of a needle.
Remove the extra glycerine using a piece of blotting paper.
Place this glass side on the stage of the compound microscope and view it.
Cheek cell:-
Gently scrape the inner side of the cheek using a toothpick, which will collect some cheek cells.
Place the cells on a glass slide that has water on it.
Mix the water and the cheek cells using a needle and spread them.
Take a few drops of Methylene blue solution using a dropper and add this to the mixture on the slide.
After 2-3 minutes remove any excess water and stain from the slide using a blotting paper.
Take a few drops of glycerine using a dropper and add this to the test mixture.
Take a clean cover slip and lower it carefully on the mixture with the aid of a needle.
Using a brush and needle, press the cover slip gently to spread the epithelial cells.
Remove any extra liquid around the cover slip using a blotting paper.
Place this glass side on the stage of the compound microscope and view it.
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