Question

prepare a cell of onion peel and find out special characters​

Answer 1

Answer:

Explanation:

Take a small piece of onion and using tweezers, peel off the membrane from the underside (the rough side). Place the membrane flat on the surface of the slide. Using a pin, lower the thin glass cover slip or cover glass onto the slide. Make sure there are no air bubbles.

Tissue from an onion is a good first exercise in using the microscope and viewing plant cells. The cells are easily visible under a microscope and the preparation of a thin section is straight forward. An onion is made of layers, each separated by a thin skin or membrane. In this exercise, you will make a wet mount on a microscope slide and look at the cells of the onion membrane magnified by the high power, compound microscope.

Answer 2

Experimental Preparation of Temporary Mounts of an Onion Peel

$\huge \bf \color{navy}Experiment:$

$\bf \color{red}Objective:$

To prepare a stained temporary mount of an onion peel and to record observations and draw labeled diagrams.

$\bf \color{red}Apparatus \: and \: materials \: required:$

An onion, glass slide, watch glass, coverslip, forceps, needles, brush, blade, filter paper, safranin, glycerine, dropper, water, and a compound microscope

$\bf \color{red}Procedure:$

1. Take an onion and remove its outermost peel.

2. Now cut a small part from an inner scale leaf with the help of a blade.

3. Separate a thin, transparent peel from the convex surface of the scale leaf with the help of forceps.

4. Keep this peel in a watch glass containing water?

5. Add two drops of safranin stain in the watch glass to stain the peel.

6. Take a clean slide and put a drop of glycerine in the centre of the slide.

7. With the help of a brush and needle transfer the peel on the slide. Glycerine prevents the peel from drying up.

8. Carefully cover it with a coverslip and avoid any air bubble from entering interring the coverslip.

9. Remove any excessive glycerine with a filter paper.

10. Observe the prepared mount of the peel under the low and high magnification of a compound microscope.

$\bf \purple{Observation:}$

A large number of rectangular cells are visible. These cells lie close to each other with intercellular spaces between them. These cells are surrounded by distinct cell walls. These cells have a dark stained nucleus and a large vacuole in the centre.

$\bf \purple{Precautions:}$

1. Overstaining and understaining should be avoided.

2. Folding of the peel should be avoided.

3. Clean and dry glass slide and coverslip should be used.

4. Coverslip should be put carefully avoiding any air bubbles.