prepare a slide for dermal tissue and rheo leaf
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First torn out the white skin of leaf and keep it in petridish with water , then add one or two drops of prepalin solution to give colour and then keep peel on the slide , put few drops of glycerine and observe under microscope
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- Take a rheo leaf,tear it in a single stroke.
- observe it against the light.
- take a small piece of leaf peel with light coloured (transparent)portion.
- Put it on slide and add 1 to 2 drops of dilute salt solution on the membrane.
- Leave it for about 5 to 10 mins and observe it under the microscope.
- Due to this water from the rheo leaf cell comes out. It results in shrinkage of the cytoplasm along with cell membrane.
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