Question

Prepare a slide of onion peel and observe the nucleus?​

Answer 1

A N S W E R :

Explanation : Onion peel experiment is done its study the cell structure.

• Glycerin is considered as a mountaing agent while making a slide of onion peel.

• It is a good hydrating agent.

• It does not allow the specimen to dry out during the long duration of study.

• lt provides a went medium for the stain so the cell structure can be easily studied.

• lt expels bubbles which may come during the maounting of the slide.

We utilize glycerin for maounting the onion peel for the following reasons –

• Glycerin which is prominently know as Glycerol helps in avoiding the lack of hydration of cells.

• Cells don't become scarce.

• As the refractive index of glycerin is 1.473 and it has a tendency to reflect. Light so the example kept looks clear under the magnifying lens.

• Glycerin is additionally used to remove air bubbles which can be found between slides.

Aim :

• To prepare a temporary stand mount of onion peel to study varius parts of the plant cells.

Materials Required :

• Onion bulb, glass slide, overslip, compound microscope, blade, watch glass, filter paper, maounting needle, water, glycerin, dropper and safranine (stain).

Procedure :

• Take the onion bulb and peel it.

• Cut it into pieces lengthwise.

• Remove a transparent peel by breaking one of the pieces into two.

• Put the peel into water in a watch glass.

• Add a drop of safranine with the help of the dropper.

• Leave the peel in the stain for a couple of minutes.

• Transfer a peel to a glass slide and with the help of a blade cut a small rectangular piece.

• Place it in the centre of the glass slide.

• Add a drop of glycerin on it.