prepare a temporary amount of any leaf , observe the stomata and draw their picture?
Answers
Answered by
0
Answer:
Experiment:
Aim
To prepare a temporary mount of a leaf peel in order to show the stomata of a leaf
Material Required
- A potted plant of Bryophyllum or Tradescantia
- Needles
- Forceps
- Watch glass
- Dropper
- Glass slides
- A brush
- Coverslips
- Blotting paper
- Safranin
- Compound microscope
- Glycerine
Procedure
- Pick a healthy leaf from the potted plant
- Fold the leaf to gently pull the peel apart to separate a peeled section from the lower surface of the leaf. Use the forceps to perform this step. Allow the peel to remain in a watch glass holding water for some time.
- In the watch glass, stain the sample by adding some drops of safranin through a dropper.
- Take the peel out after 2-3 minutes. Set it on a clear glass slide
- Add a drop of glycerin on the peel. Put a clear coverslip over it gently using a needle.
- Excess glycerin and stain can be removed using blotting paper
- Examine the slide first under a low-power and then under a high-power magnification of a compound microscope.
Observation
- Visible epidermal cells. The cells in their outline are irregular with no intercellular spaces
- Small openings, stomata are scattered through the epidermal cells
- Guard cells are observed which have chloroplasts and nucleus
- Guard cells are observed having a thin outer covering and a thick inner boundary(concave)
- Guard cells control the closing and opening of the stomata
Conclusions
Epidermal cells are found containing stomata on the lower surfaces of the leaf.
Similar questions