Question

prepare a temporary Mount of any leaf peel observe stomata Android A picture write a short note on same

Answer 1

Requirements ...
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Leaf of a plant like lily, Bryophyllum , or tradenscantia, forceps, waych glass, slide, coverslip , brush, needles, safranin, glycerine, and microscope .

Principal Involved ...
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Stomata are minute appertures ( openings ) present on the surface of the leaf . they are generally more on the lower epedermis . Each stomata is surrounted by two bean-shaped cells called “gaurd cells” . Theae cells have differential thickenings, i.e. thicker inner wall facing the stoma and thinner outer wall . The turgidity of gaurd cells controls the opening and closing of stomata . Stomata heps in exchange of gases and water vapours between the atmosphere land leaf .

Procedure ...
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1 ) Pell off the leaf from its under surface and put the peel in a watch glass containing water .
2 ) Add a few drops of Safranin stain into the watch glass to stain the peel .
3 ) After staining put the peel on the cleaning slide and cut it in a rectangular or square shape .
4 ) With the help of a filter paper, remove the excess of stain and water .
5 ) Put a drop of glycerine on the slide over the peel, gently put the coverslip to avoid the entry of air bubbles and observe under the microscope .

Observations ...
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1 ) Epidermis consists of a single layer of cells which are irregular in outline and without intracellular spaces .
2 ) Minute appertures ( openings ) are seen which are imbedded in epidermal cells .

Result ...
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The minute apperture seen in the mount are stomata . Each stomata is surrounded by two bean-shaped cells called gaurd cells .

Precautions ...
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1 ) The epidermal peel should be taken from a freshly plucked leaf .
2 ) Always hold the slide by its edges to avoid making the slide dirty .
3 ) Always use a brush to transfer the peel from petri dish to the slide .
4 ) The peel should be cut to a proper size and its curling must be avoided .
5 ) The peel should be mounted in the centre of the slide .

Answer 2

1. The epidermal cells are visible. These are irregular in outline and have no intercellular spaces.  2. Many small pores (stomata) are seen scattered among the epidermal cells.  3. Each pore is guarded by two bean-shaped guard cells, each containing chloroplasts and a nucleus. 4. The inner concave boundary of each guard cell is thick, whereas its outer boundary is thin.