Question

prepare a temporary Mount of leaf peel observe the tomato and draw their picture write a short note on the same​

Answer 1

Answer:

Hope it helps you......(✿◕‿◕✿)

Explanation:

AIM:-

To prepare a temporary mount of leaf peel to show its stomata

Requirements:-

Leaf of a plant like lily, Bryophyllum , or tradenscantia, forceps, watch glass, slide, coverslip , brush, needles, safranin, glycerin, and microscope .

Principal Involved:-

Stomata are minute apertures ( openings ) present on the surface of the leaf. They are generally more on the lower epidermis . Each stomata is surmounted by two bean-shaped cells called “guard cells” . These cells have differential thickenings, i.e. thicker inner wall facing the stoma and thinner outer wall . The turgidity of guard cells controls the opening and closing of stomata . Stomata helps in exchange of gases and water vapours between the atmosphere land leaf .

Procedure:-

1) Pell off the leaf from its under surface and put the peel in a watch glass containing water .

2) Add a few drops of Safranin stain into the watch glass to stain the peel .

3) After staining put the peel on the cleaning slide and cut it in a rectangular or square shape .

4) With the help of a filter paper, remove the excess of stain and water .

5) Put a drop of glycerin on the slide over the peel, gently put the coverslip to avoid the entry of air bubbles and observe under the microscope .

Observations:-

1 ) Epidermis consists of a single layer of cells which are irregular in outline and without intracellular spaces .

2 ) Minute apertures ( openings ) are seen which are imbedded in epidermal cells .

Result:-

The minute aperture seen in the mount are stomata . Each stomata is surrounded by two bean-shaped cells called guard cells .  

Precautions:-

1 ) The epidermal peel should be taken from a freshly plucked leaf .

2 ) Always hold the slide by its edges to avoid making the slide dirty .

3 ) Always use a brush to transfer the peel from petri dish to the slide .

4 ) The peel should be cut to a proper size and its curling must be avoided .

5 ) The peel should be mounted in the center of the slide .

6 ) The peel should not be allowed to dry .

7 ) Always keep the coverslip gently to avoid the entry of air bubbles .

8 ) Oozing of glycerin should not be there .

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Answer 2

Answer:

$\huge\red{Aɴsᴡᴇʀ:-}$

Aɪᴍ:-

Tᴏ ᴘʀᴇᴘᴀʀᴇ ᴀ ᴛᴇᴍᴘᴏʀᴀʀʏ ᴍᴏᴜɴᴛ ᴏғ ᴀ ʟᴇᴀғ ᴀɴᴅ ᴛᴏ ᴏʙsᴇʀᴠᴇ ᴛʜᴇ ᴄᴇʟʟ ᴏʀɢᴀɴᴇʟʟs.

Mᴀᴛᴇʀɪᴀʟs ʀᴇǫᴜɪʀᴇᴅ:-

A ʟᴇᴀғ,ᴡᴀᴛᴄʜ ɢʟᴀss,ᴄᴏᴠᴇʀ sʟɪᴘ,ᴍɪᴄʀᴏsᴄᴏᴘᴇ,ᴍᴇᴛʜʏʟᴇɴᴇ ʙʟᴜᴇ,ɢʟᴀss sʟɪᴅᴇ,ɢʟʏᴄᴇʀɪɴᴇ.

Pʀᴏᴄᴇᴅᴜʀᴇ:-

1. Tᴀᴋᴇ ᴀ ʟᴇᴀғ ᴀɴᴅ ᴛᴇᴀʀ ɪᴛ.Pᴜᴛ ᴀ sᴍᴀʟʟ ᴘᴏʀᴛɪᴏɴ ᴏғ ᴛʜᴇ ʟᴇᴀғ ᴏɴ ᴀ ᴡᴀᴛᴄʜ ɢʟᴀss.
2. Tʀᴀɴsғᴇʀ ᴛʜᴇ ᴄᴏɴᴛᴇɴᴛs ᴛᴏ ᴛʜᴇ ɢʟᴀss sʟɪᴅᴇ ᴀɴᴅ ᴀᴅᴅ ᴍᴇᴛʜʏʟᴇɴᴇ ʙʟᴜᴇ ᴛᴏ ɪᴛ.
3. Dʀᴀɪɴ ᴏᴜᴛ ᴛʜᴇ ᴇxᴛʀᴀ sᴛʀᴀɪɴ ᴡɪᴛʜ ᴀ ʙʟᴏᴛᴛɪɴɢ ᴘᴀᴘᴇʀ.
4. Pᴜᴛ ᴀ ᴅʀᴏᴘ ᴏғ ɢʟʏᴄᴇʀɪɴᴇ ᴏᴠᴇʀ ɪᴛ ᴀɴᴅ ᴘʟᴀᴄᴇ ᴛʜᴇ ᴄᴏᴠᴇʀsʟɪᴘ.Tᴀᴋᴇ ᴄᴀʀᴇ ᴛʜᴀᴛ ᴛʜᴇʀᴇ ᴀʀᴇ ɴᴏ ᴀɪʀ ʙᴜʙʙʟᴇs ᴡʜɪʟᴇ ᴘʟᴀᴄɪɴɢ ᴛʜᴇ ᴄᴏᴠᴇʀsʟɪᴘ.
5. Pʟᴀᴄᴇ ɪᴛ ᴜɴᴅᴇʀ ᴛʜᴇ ᴍɪᴄʀᴏsᴄᴏᴘᴇ ᴀɴᴅ ᴏʙsᴇʀᴠᴇ.

Oʙsᴇʀᴠᴇ:-

Wᴇ ᴄᴀɴ ᴏʙsᴇʀᴠᴇ ᴛʜᴇ ᴄᴇʟʟ ᴡᴀʟʟ,ᴄʜʟᴏʀᴏᴘʟᴀsᴛs ᴀɴᴅ ᴀ ʟᴀʀɢᴇ ᴠᴀᴄᴜᴏʟᴇ ᴡʜɪᴄʜ ᴏᴄᴄᴜᴘɪᴇs ᴀʟᴍᴏsᴛ ᴛʜᴇ ᴇɴᴛɪʀᴇ sᴘᴀᴄᴇ ᴏғ ᴛʜᴇ ᴄᴇʟʟ.Tʜᴇ ᴄᴇʟʟ ᴏʀɢᴀɴᴇʟʟᴇs ᴀʀᴇ sᴇᴇɴ ᴀᴛ ᴏɴᴇ sɪᴅᴇ ᴏғ ᴛʜᴇ ᴄᴇʟʟ.