Prepare a temporary mount of onion root tip to study mitosis
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Answer:
sorry bro this answer is to long
Procedure to prepare a temporary mount of onion root tip to study different stages of mitosis like prophase, metaphase, anaphase and telophase, are as follows:-
1. Take an Onion and place it on the tile.
2. Remove extra dry roots by using a blade.
3 With the help of forecep transfer them to freshly prepared fixative of aceto alcohol.
4. Keep the root tips in fixative fir 24 hours and using forecep take one root and place it on clean slide.
5. Using a dropper place one drop of N/ 10 HCL on trot tips then add again 2-3 drops to it.
6. Warm it by using burner and make sure that the stain wouldn't get dry.
7. Carefully blot the excess stain by using filter paper.
8. Now cut comparatively more stained tip portion of the root retain it on the slide and discard the remaining portion.
9. Now add drop of water on the root tip.
10. Mount cover slip on it using a needle.
11. Now slowly tap the cover slip using the blunt end of a needle.
11. This prepared onion root tip is now ready for the study of mitosis.
12. We can place slide now under the compound microscope and can observe the different stages of mitosis.