Question

Prepare a temporary mount of the leaf to
show the presence of stomata.​

Answer 1

Procedure :-

1. Pick a healthy leaf from the potted plant
2. Fold the leaf to gently pull the peel apart to separate a peeled section from the lower surface of the leaf. Use the forceps to perform this step. Allow the peel to remain in a watch glass holding water for some time.
3. In the watch glass, stain the sample by adding some drops of safranin through a dropper.
4. Take the peel out after 2-3 minutes. Set it on a clear glass slide
5. Add a drop of glycerin on the peel. Put a clear coverslip over it gently using a needle.
6. Excess glycerin and stain can be removed using blotting paper
7. Examine the slide first under a low-power and then under a high-power magnification of a compound microscope.