prepare a temporary mount to observe poller germination?
Answers
Explanation:
Procedure: Prepare a nutrient solution by dissolving 10g sucrose and 10 mg boric acid in 100 ml water. Take a few drops of this solution on a clear slide, dust a few pollen grains from the stamen of a mature flower in it. Obverse the slide under the microscope after 5 minutes.
A pollen grain was considered germinated when pollen tube length was at least equal to or greater than the grain diameter [15]. Germination percentage (%) was determined by dividing the number of germinated pollen grains per field of view by the total number of pollen per field of view
Answer:
Pollen germination can be studied by dusting some pollens from common flowers like Crotalaria, Hibiscus, Pisum, etc. on a glass slide containing a drop of 10% sugar solution or tender coconut water or any nutrient medium. Observe the slide after about 10 – 15 minutes under the low power of compound microscope.