prepare a temporary stained slide of the given root trip to study mitoses. Draw a labelled diagram of your preparation and comment on it.
Answers
Answer:
Place the onion on a tile
Using the blade, remove the dry roots
Regrow the root tips by placing the bulbs in a water-filled beaker
After 3 to 6 days, new roots may emerge
Slide 2 to 3 cm off freshly grown roots and place them on a watch glass
Use a forceps to transfer the freshly cut tips to a test tube containing Aceto-alcohol (1:3 = anhydrous acetic acid: ethanol)
Submerge the root tips in the solution for 24 hours
Use the forceps to take out a single root and place it on a glass slide.
Put a single drop of N/10 HCl on the root tip
Then, put 2-3 drops of acetocarmine stain
Use a burner to warm it, and ensure that the stain does not dry up.
Use a filter paper to blot out the excess stain, if any
Cut the significantly stained portion of the root using a blade and place it on a slide. Discard the rest of the root
Put a drop of water on the root tip
Place a coverslip using a needle
Tap the coverslip such that the meristematic tissue of the root tip is compressed and spread out as a thin layer.
The preparation is ready for studying mitosis.