prepration of permanent slides of pollon grain by acetolysis method principle and material
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Add about 6 ml of acetolysis mixture (acetic anhydride and conc. sulphuric acid in a ratio of 9 : 1) over the pollen grains in tube 'B', place it in a water bath and heat the water from 70°C to 100°C. When the water starts boiling, stop the flame and leave this centrifuge tube 'B' in hot water for 3-5 minutes.
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