Principle and application of flow cytometry
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General Principles. Flow cytometry measures optical and fluorescence characteristics of single cells (or any other particle, including nuclei, microorganisms, chromosome preparations, and latex beads).When a cell suspension is run through the cytometer, sheath fluid is used to hydrodynamically focus the cell suspension through a small nozzle. ... Fluorescence detectors measure the fluorescence emitted from positively stained cells or particles.
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