Principle and procedures of histological staining in nucleic acid
Answers
Answer:
Histochemistry is an important technique that is used for the visualization of biological structures. As such, it is concerned with the identification and distribution of various chemical components of tissues through the use of stains, indicators as well as microscopy.
Explanation:
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As the nucleic acids - DNA and RNA, are obviously acidic, basic stains have to be used in their histological staining procedures.
The common stains:
1. Methyl Green-Pyronin (MGP) stain
- MGP is the classical histological staining technique. Methyl green is specific for DNA and Pyronin for RNA.
2. Feulgen stain.
- This is used for the demonstration of DNA giving a red colour to it.
Procedure using MGP stain:
(Fixed paraffin embedded tissue samples are typically used.)
1. De-paraffinize slide using Xylene or any other Substitute
2. hydrate through alcohols.
3. Rinse slide in running Tap water and then with distilled water.
4. Keep the slide in MGP stain in room temperature for 2 to 7 minutes.
5. Dip slide 1 to 2 times each through 2 changes of distilled water in room temperature.
6. Dehydrate slide through 3 changes of fresh Reagent alcohol.
7. Clear slide through 3 changes of fresh Xylene.
8. Place the cover slip using a permanent mounting medium.
INFERENCE:
DNA: BLUE-GREEN TO GREEN
RNA: PINK TO RED
NOTE:
- Longer stain time intensifies Pyronin (Red); shorter stain time intensifies Methyl Green (Blue-green to green) .
Know more:
What is meant by staining ???
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Name the two nucleic acids present in the cell and their functions.
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