Principle for isolation of genomic dna from gram negative bacteria
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Principle behind DNA isolation and roles of reagents used in isolation. ... GRAM NEGATIVE VS GRAM POSITIVE - LYSIS The ... “E. Coli Genomic DNA Extraction.
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The isolation of DNA from bacteria, described in this protocol, relies upon the use of SDS and proteinase K to lyse the cells. High-molecular-weight DNA is then sheared (to reduce its viscosity and make it more manageable), extracted with phenol:chloroform, and precipitated with isopropanol.
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