Biology, asked by nivekitty10361, 10 months ago

Principle of coomassie brilliant blue r250 staining solution

Answers

Answered by mpssankar99

Answer:

When Coomassie Brilliant Blue G-250 binds to proteins in acid solution, it has an absorbance shift from 465 nm to 595 nm. The absorbance data can then be used in Beer's law to determine protein concentration and ultimately the actual amount of protein in a given solution.

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