Question

Principle of ctab method for dna isolation

Answer 1

The use of CTAB (cetyl trimethylammonium bromide), a cationic detergent, facilitates the separation of polysaccharides during purification while additives, such as polyvinylpyrrolidone, can aid in removing polyphenols. CTABbased extraction buffers are widely used when purifying DNAfrom plant tissues.
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Answer 2

Principle of ctab method for dna isolation?

Answer:

CTAB (Cetyltrimethyl ammonium bromide) is a detergent used to break open plant cells and solubilize its contents. ... This is followed by disruption of the cell membrane to release the DNA into the extraction buffer. The released DNA should be protected from endogenous nucleases.