Principle of histochemical stain biology discussion
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Answer:
Place the deparaffined plant tissue sections in 0.5% aqueous HIO4 for 10-15 min at room temp.
2. Wash in running water for 10 min.
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3. Place in Schiff’s reagent for 15-20 min (Schiff’s reagent is prepared by dissolving 0.5 g of basic function and 0.5 g of sodium meta-bisulfite in 100 ml 100 ml 0.15 N HCl. Shake the mixture for 2-3 hrs. Add 30 mg fresh decolorizing charcoal and shake for 5 min.
Answer:
we will discuss about the application of some histochemical methods in the study of plants: 1. Procedure for Histochemical Localization of Total Insoluble Polysaccharides 2. Histochemical Localization of Starch by 1 Kl Method 3. Histochemical Localization of Proteins by Bromophenol Blue Method and few others.
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