Question

Principle of using mercuric bromophenol blue for total protein

Answer 1

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$\implies$ Bromophenol blue is used as a pH indicator.

$\implies$The interactions of Bromophenol Blue (BPB) with bovine serum albumin and γ- globulin in acidic solutions.

$\implies$Bromophenol Blue (BPB) Protein Bromophenol blue (BPB), a phenolphthalein anionic dye That binds to proteins under neutral to acidic condition.

Answer 2

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its is a phenolphthalein anionic dye, binds to proteins under neutral to acidic conditions. From this attribute, BPB can determine protein levels in samples, especially in solubilized cultured cells and cell membrane proteins with high concentrations of surfactants . This is an advantage over the widely used coomassie blue protein assay which is affected by the presence of surfactants in the samples.

Proteinuria, the presence of an excess of serum proteins in the urine, is a useful marker of renal disease. In addition, the pathophysiology of proteinuria can be divided into tubular or glomerular dysfunction.

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