Principles and applications of ion exchange, membrane separation processes and chromatography
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Principle of Ion Exchange Chromatography. IEX chromatography is used in the separation of charged biomolecules. The crude sample containing charged molecules is used as the liquid phase. When it passes through the chromatographic column, molecules bind to oppositely charged sites in the stationary phase.
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An important use of ion-exchange chromatography is in the routine analysis of amino acid mixtures.Ion exchange is used for both analytical and preparative purposes in the laboratory, the analytical uses being the more common.
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