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principles of acid fast staining

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Acid-Fast Stain- Principle, Procedure, Interpretation and Examples. It is the differential staining techniques which was first developed by Ziehl and later on modified by Neelsen. ... Neelsen in 1883 used Ziehl's carbol-fuchsin and heat then decolorized with an acidalcohol, and counter stained with methylene blue.
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