Question

Procedure to determine the amount of drug present in a solution

Answer 1

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Answer 2

Explanation:

The area under the curve which you get from the above dilution is your concentration of 5 mg in 5 ml and not 10 ul because 10 ul is your injection volume where as the actual concentration is 5 mg in 5 ml.

You can find out the concentration of unknown amount of drug by injecting a reference standard solution of the known concentration. Prepare a standard solution of the same drug of known concentration and inject it into the HPLC system. The area under the curve which you get for your standard sample can be used to calculate the concentration of drug of unknown concentration.