Question

PROCESS OF ELISA TEST IN SIMPLE WAY ☆☆ DONT SPAM​

Answer 1

Explanation:

Primary sample antibody is added to the plate and incubated with the antigen. The plates are washed with PBS to remove unbound molecules. The secondary enzyme conjugated antibody is added and incubated with the antigen. Chromophore substrate is added which detects the presence of the enzyme and thus the antigen.