Question

Process of microbiological assay of amino acids

Answer 1

Answer:

The use of microbiological tube assays requires attention to many details not ordinarily encountered in analytical chemistry. The microorganism and the medium in which it grows are used as reagents. Considerations in the study, development, and application of microbiological tube methods include reference standards, test organisms, media, sample preparation, assay tube preparation, measurement of response, and technique. Reference standards should be pure and authentic, test organisms should be sensitive to the substance under test, the media should be of known composition, the sample should be measured precisely and prepared so as to liberate the active component and eliminate interfering substances, the assay tubes should be prepared without harming the sample, and the measurement of response should be done accurately by titrimetry, turbidimetry, gravimetry, or other suitable technique.

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Answer 2

Answer:

A microbiological plate method, with Sarcina Zutea as test organism, is described for the assay of penicillin in feeding stuffs. The method is capable of determining penicillin in samples containing less than 5 units per g, at which level plate assay methods with Bacillus subtilis are insufficiently sensitive.