Production of disease free plants through tissue culture procedure
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1)a small piece of plant tissue is taken from the meristematic regions or growing reasons for example root or shoot tips of the plant and is transferred to a test tube or flaskhaving suitable nutrient media under aseptic conditions .
2)the tissue placed over culture medium divide rapidly due to presence of suitable hormones and form an undifferentiated mass of cells called callus.
3)small pieces of the callus transferred into another cellular medium having suitable growth hormones like auxins and cytokinins to stimulate growth of roots and shoots.
4)the callus differentiate into plantlets
5)these plantlets are transferred into ports for soil to grow and attain maturity
2)the tissue placed over culture medium divide rapidly due to presence of suitable hormones and form an undifferentiated mass of cells called callus.
3)small pieces of the callus transferred into another cellular medium having suitable growth hormones like auxins and cytokinins to stimulate growth of roots and shoots.
4)the callus differentiate into plantlets
5)these plantlets are transferred into ports for soil to grow and attain maturity
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