Question

production of fungal proteins by filamentous fungi using strain improvement techniques

Answer 1

Filamentous fungi have been used as sources of metabolites and enzymes for centuries. For about two decades, molecular genetic tools have enabled us to use these organisms to express extra copies of both endogenous and exogenous genes. This review of current practice reveals that molecular tools have enabled several new developments. But it has been process development that has driven the final breakthrough to achieving commercially relevant quantities of protein. Recent research into gene expression in filamentous fungi has explored their wealth of genetic diversity with a view to exploiting them as expression hosts and as a source of new genes. Inevitably, the progress in the ‘genomics’ technology will further develop high-throughput technologies for these organisms.

Answer 2

here is ur answer mate...
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.During the last 5 years, strain improvement by genetic engineering has been established as a good alternative for traditional methods of strain improvement such as mutagenesis and genetic recombination. Considerable success has been achieved in the overproduction of a variety of fungal proteins. However, from the available data a limitation at the level of transcription is evident. A more detailed analysis of this transcription limitation was carried out for the overproduction of glucoamylase in A. niger. This analysis revealed that both the site of integration of introduced gene copies and the available amount of trans-acting regulatory proteins were at the root of the observed limitation. Based on these results, approaches for the construction of a new generation of protein-overproducing strains are suggested.....
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