Question

Protein x is 1000aa and mw of gfp is 27 kda total mw of fusion protein in dalton

Answer 1

Answer:

But notice that, some need protein AA sequence and some need importing data from UniProt Knowledgebase (Swiss-Prot or TrEMBL),...

Finally it may be post traslational modifications changed the MW.

So it shows only stimate of MW related to fused proteins.

And you can check it experimentally by Gel filtration, or electrophoresis too.

Answer 2

Answer:

I fused the GFP to my protein of interest. I know their MW separately, but I would like to know the MW of my protein with GFP. I know there are some websites .

Hope this help u ❤