Put a black spot using a sketch pen on one end of a filter paper cut like a ribbon. Keep
this dipped in the water in a beaker as shown in figure 1.
a) What change can be observed for the black spot in figure 2?
b) What is your inference?
c) Name the method for separating components from a mixture in this way
Answers
Answer: YOUR ANSWER
Explanation: CHROMATOGRAPHY SEPARATION
The spot of ink on the filter paper starts to move upwards by capillary action.
The water makes the ink travel upward separating its different pigments on the filter paper,
This phenomenon of capillary movement taking place by the ink spot on the filter paper is known as Ink Chromatography Separation.
EXPLANATION:
Chromatography is a laboratory technique used to separate two or more different components of the same material. For that, the substance which is supposed to be separated into components is mixed with a liquid that acts as a mobile phase. Mostly chromatography is carried out on filter papers where the mobile phase helps the component to move upward with the ability of capillary movement. The different components of the substance start to move with their specific velocity and move up to their capabilities indicating the presence in a particular quantity. This method of separation is widely used for protein purification and its usage is commonly found for the same. The substance that undergoes chromatography will always have two phases that are:-
MOBILE PHASE: The part of the substance that travels through the filter paper.
STATIONARY PHASE: The part of the substance that stands still and does not show any movement.