Q.1 how you will prepare 4% paraformaldegyde solution?
how you will prepare the solutions?
solution 1 ( 500mL; pH 8.5)
1% Tryptone + 0.5% yeast extract + 200mM NaCl.
solution 2(500mL;pH 8.8)
50mM glucose + 10mM EDTA + 25mM tris (pH 8.0)
plz answer
Answers
Answer:
Formaldehyde is a simple aldehyde (equivalent of a monomer to paraformaldehyde) with formula CH2O.
Formalin, on the other hand, is a saturated solution of formaldehyde (37%). 10% formalin is equivalent to 4% formaldehyde. However, many vendors use a small amount of methanol or other chemicals to prevent polymerization of formaldehyde in the solution. These additional reagents must be considered as they may yield unwanted effects.
Paraformaldehyde (PFA) is a polymer of formaldehyde with 8-100 units.
Paraformaldehyde, when dissolved in water, breaks down into formaldehyde. This solution differs from commercially available form (formalin) being relatively pure (devoid of methanol). In immunohistochemistry (IHC) and cell biology experiments, researchers prefer working with PFA solution rather formalin due to the same reason.
Although formalin and paraformaldehyde solutions said to be having formaldehyde, formaldehyde in these solutions is hydrated and converts (most of the formaldehyde) into methylene glycol. In these solutions (formalin or paraformaldehyde), a major portion of methylene glycol is in equilibrium with formaldehyde. However, only formaldehyde (not methylene glycol) have cross-linking ability.
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