Question

Q. 1) Select and write the correct answer (one mark each) 08 1) During denaturation step of polymerase chain reaction, the reaction mixture is heated at 01 a) 40-60°C b) 70-75°C c) ) 90-98°C d) 36°c 01 ​

Answer 1

Answer:

40°to 60°

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Answer 2

Answer:

C) 90-98°C

Explanation:

Denaturation is the process in which molecules break down due to high temperature or strong acid/base.

A polymerase chain is a chain of monomers (subunits of organic molecules).

I think that the denaturation the question is mentioning is most likely to be about nucleic acids (DNA).

The highest temperature available to choose is 90-98°C. It's usually at 95°C that the hydrogen bonds linking the nitrogenous bases break down during PCR.