Q.15 :-The Vmax of an enzyme represents :
The amount of active enzymes
O
Substrate concentration
Half the substrate concentration
Total concentration of the enzyme
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In the simplest case of a monomeric enzyme with a single active site, the Km is independent of the enzyme concentration, in principle. However, if the measurement is not done under the right conditions for Michaelis-Menten kinetics, the Km may appear to vary with the enzyme concentration. The enzyme concentration must be much lower then the substrate concentration, and you must measure the initial rate of the reaction. If the enzyme concentration is too high, these conditions may be violated.
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