Question

Q.22. Which region codes for proteins involved in the uptake and metabolism of opines in Ti plasmids?

Answer 1

Answer:

Opines are low molecular weight compounds found in plant crown gall tumors or hairy root tumors produced by pathogenic bacteria of the genus Agrobacterium and Rhizobium. ... Each strain of Agrobacterium and Rhizobium induces and catabolizes a specific set of opines, this set typifying theTi plasmid and Ri plasmid.

Answer 2

Answer:

• Agrobacterium tumefaciens is a Gram-negative, rod-shaped, motile bacterium. In many dicots, the bacterium causes crown gall disease (tumor-like growth in the crown region).
• A plasmid called Ti plasmid (tumour inducing plasmid) is found in Agrobacterium tumefaciens tumour cells, and it integrates a tumor-causing gene (tRNA) Ti plasmid was introduced into the host genome, causing gall formation in the crown region.
• It Is widely used in genetic engineering and is currently used in the genetic modification of plants such as cotton, tomatoes, potato, rice, maize, and so on.
• The Ti plasmid's vir locus encodes the genes required for bacterial conjugation. Opines are not metabolised as a nutrient source by plants or most organisms. Agrobacterium produces opiates, which are then transported to the plant cell.

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