Question

Q.31. Which of the following is the correct Line weaver-Burk equation?
a. 1V0=KmVmax[S]+1Vmax
b. 1Vmax=KmV0[S]+1V0
c. VO=Vmax[S]Km+[S]
d. Vmax=VO[S]Km+[S]​

Answer 1

Answer:

c is the correct answer i think ur options are quite wrong

Explanation:

VO=Vmax[S]/Km+[S]

Answer 2

The correct Line weaver-Burk equation is 1/V₀ = Km/Vmax [S] + 1/Vmax (option - a)

• Michaelis Menten equation gives the rate of an enzyme-catalyzed reaction catalyzed for a single substrate.
• V₀ = Vmax [S]/(km + [S]) → Michaelis Menten equation.
• The graph between velocity of enzyme catalysed reaction and substrate concentration is represented as a hyperbola.
• The rate of reaction increases directly with increase in substrate concentration[S] and reaches a maximum velocity i.e. Vmax after which the rate remains constant with an increase in the substrate concentration as all enzymes are saturated i.e. bound by a substrate.
• Km is defined as the substrate concentration at which the rate of reaction is half Vmax.
• For easy visualization as a straight line, a Line weaver-Burk plot is used.
• The Line weaver-Burk equation is the reciprocal of the Michaelis Menten equation ⇒ 1/V₀ = Km/Vmax [S] + 1/Vmax