Question

Q.If DNA is synthesized in an in vitro reaction containing equimolar amounts of substrates (adenine, thymine,
guanine and cytosine) from a template with [A+G/C+T) = 0.97, the value of (A+G/C+T) in the product will
be close to
a. 0.25
b. 0.5
C. 0.8
d. 1.01

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Answer 1

Answer:

A + T/G + C ratio is species specific and here mentioned to be 0.3.

While A + G/T + C is the ratio which is constant for all double-stranded DNA and its value is 1 because in a ds-DNA Adenine will always bind to Thymine so A=T while Guanine will always bind to Cytosine so G=C and Purine will always be equal to pyrimidines according to Chargaff rule.

So, the correct answer to the question is '1'.