Question

Q.No.4. Describe the critical steps required to make a recombinant bacterial cell for production
of commercial products.
Marks=1)
Answer:​

Answer 1

Which is a step in the process of producing recombinant bacteria?

1. Isolate DNA from two sources.
2. Cut both DNA with some restriction enzyme.
3. Mix DNA they join by base pairing.
4. Add DNA ligase to bond covalently.
5. Put plasmid into bacterium by transformation.
6. Identify cells containing recombinant plasmid by ability to grow in presence of ampR and tetR.
7. Clone selected bacteria.

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