Question

Q} What precaution should be taken while bleaching the leaf in the experiment to show that Chlorophyll is essential for photosynthesis ? GIVE REASON
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PLS GIVE THE CORRECT ANSWER... URGENT​

Answer 1

Answer:

leaf should be boiled appropriately in alcohol to drain all its chlorophyll (green colour)

Answer 2

Answer:

First you are supposed to destarch the plant by keeping it in the dark room for few days.

Then you are supposed to place the plant in the sun and after few hours pluck a leaf.

Mark its outline on paper and mark the green and non green parts on the outline.

Then test the leaf for starch only the green parts of the leaf will turn into blue black whereas the non green parts will turn brown

To test the leaf for starch:

• Dip the leaf in boiling water for a few minutes to kill the cells.

• Boil the leaf in methylated spirit over a water bath till it becomes pale white die to removal of chlorophyll.

• The leaf now turns brittle and hard place the leaf in hot water to soften it.

• Spread the leaf in a dish and pour iodine solution on it. The presence of starch will be indicated by a blue black colour. A leaf without starch will show brown colour.

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