Question

Q1.An enzymologist has isolated and purified an enzyme M. To check the
activity of an enzyme he performed several different experiments.


(I) With the approach of site directed mutagenesis, to create a targeted
change in DNA, to study the changes in protein activity. Change in the
codon ACT→GCT, how it affects the activity of the enzyme? Comment
(Note: Amino acid lies in the active site of the enzyme, plays a role in
catalysis)

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Answer 1

Answer:

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Answer 2

Answer:

Explanation: Km is defined as the concentration of substrate at which enzyme is working at half of maximum velocity. It is also a measure of the affinity that the enzyme has for its substrate. Michaelis-Menten kinetics assumes non-covalent binding between enzyme and substrate.