Question

Q55. Polynucleotide phosphorylase is the enzyme which is used in this process? पॉली न्यूक्लियोटाइड फॉस्फोराइलेस वह एंजाइम है जिसका उपयोग इस प्रक्रिया में किया जाता है? A. Transcription B. Translation C. Polymerization D. Digestion​

Answer 1

Answer:

bifunctional enzyme with a phosphorolytic 3' to 5' exoribonuclease activity and a 3'-terminal oligonucleotide polymerase activity.[2] That is, it dismantles the RNA chain starting at the 3' end and working toward the 5' end.[1] It also synthesizes long, highly heteropolymeric tails in vivo. It accounts for all of the observed residual polyadenylation in strains of Escherichia coli missing the normal polyadenylation enzyme.[1] Discovered by Marianne Grunberg-Manago working in Severo Ochoa's lab in 1955, the RNA-polymerization activity of PNPase was initially believed to be responsible for DNA-dependent synthesis of messenger RNA, a notion that got disproved by the late 1950s.[3][4]