Quantitative and qualitative aspects of chromatographic methoda of analysis
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how to perform quantitative measurement using chromatographic separation techniques. It discusses the different methods of calibration such as the external standard method, the internal standard (IS) method, the standard addition method, and normalization. The chapter also presents a short introduction to qualitative analysis using chromatographic separation techniques combined with mass spectrometry and nuclear magnetic resonance spectroscopy. Quantitative measurements using chromatographic analysis are based on measurements of peak height or peak area of the peak from a sample with the unknown concentration. The analytical method should always be validated before use. The use of qualitative analysis in bioanalytical chemistry is typically concerned with the identification of organic molecules in samples of biological origin. In drug discovery, it is important to know the metabolites formed in the different animal species and in humans used in the R&D process.