Quantitative measure of a column’s ability to separate two solutes is called ---------------- a)Partition d)coefficient resolution c)dead time d)HETP
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The quantitative measure of a column’s ability to separate two solutes is called partition coefficient (option-a).
- Chromatography is a technique that is used to separate two or more components of a mixture.
- It consists of a mobile phase and a stationary phase.
- The mobile phase can be liquid in the state or gaseous in the state.
- The stationary phase can be a liquid or a solid.
- The mobile phase and stationary can be non-polar or polar in nature.
- In column chromatography, components of a mixture are separated on the basis of their polarity.
- On the basis of differential partitioning between the mobile phase and stationary phase, two solutes are separated.
- This ability of the column to separate two solutes is called the partitioning coefficient.
- It can be written as-
Partitioning coefficient, K = concentration in stationary phase / concentration in mobile phase
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