que .1 note on sub cutting of bacteria
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Bacterial are unicellular prokaryotic organism. Bacterial cell have simpler internal structure. It lacks all membrane bound cell organelles such as mitochondria, lysosome, golgi, endoplasmic reticulum, chloroplast, peroxisome, glyoxysome, and true vacuole. Bacteria also lacks true membrane bound nucleus and nucleolus. The bacterial nucleus is known as nucleoid.
A typical bacterial cell have following structure.
A. Structure Outside cell wall
Capsule
Flagella
Pili
Sheath
Prostheca
Stalks
B. Structure Inside cell wall
Cell wall
Cytoplasmic membrane
Nucleoid
Mesosome
Ribosome
cytoplasm
Spore
1. Capsule:
Capsule is 0.2µm thick viscus layer outer layer to the cell wall.
Capsule is 98% water and 2% polysaccharide or glycoprotein/ polypeptide or both.
There are two types of capsule.
i. Macro-capsule: thickness of 0.2µm or more, visible under light microscope
ii. Microcapsule: thickness less than 0.2µm, visible under Electron microscope
Capsule is very delicate structure. It can be removed by vigorous washing.Capsule is most important virulence factor of bacteria.
Function:
It helps in attachments as well as it prevent the cell from desiccation and drying.
Capsule resist phagocytosis body
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