Que of std 12 ( bio ) - how did Hershey and chase differentiate between DNA and protein in their experiment while proving that DNA is the genetic material ?
Answers
Explanation:
it is clear that debate between proteins versus DNA as genetic material was an equally resolved by hershey and chase experiment it became an established fact that it is DNA the tax is genetic material however it subsequently became clear that in some virus RNA is genetic material for example tobacco mosaic virus qb bacteriophage HIV accept the answers to some questions answers why DNA is predominant genetic material virus RNA performs annual functions of messenger and adaptor can be found from differences between chemical structures of Tu nikalke acid molecules
Answer:
Hershey and Chase worked with bacteriophage and E.coli to prove that DNA is the genetic material. They used different radioactive isotopes to label DNA and protein coat of the bacteriophage. They grew some bacteriophages on a medium containing radioactive phosphorus (32P) to identify DNA and some on a medium containing radioactive sulphur (35S) to identify protein. Then, these radioactive labelled phages were allowed to infect E.coli
bacteria. After infecting, the protein coat of the bacteriophage was separated from the bacterial cell by blending and then subjected to the process of centrifugation. Since the protein coat was lighter, it was found in the supernatant while the infected bacteria got settled at the bottom of the centrifuge tube. Hence, it was proved that DNA is the genetic material as it was transferred from virus to bacteria.
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