Question 20 : Separation of
components of mixture in column
chromatography depends on......
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The principle of column chromatography is based on differential adsorption of substance by the adsorbent. ... The rate at which the components of a mixture are separated depends on the activity of the adsorbent and polarity of the solvent.
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Column Chromatography is an analytical technique in which column packed with a solid, which serves as a stationary phase, and the liquid (the mobile phase or eluent) runs through this column. Separation of the mixture depends upon the strong affinity for the adsorbent.
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