Question

Question(Note)
Incorrect answers will deduct from the points for this


Which four of the following answers are possible mechanisms for translational inhibition that is mediated by miRNA-RISC?

RISC may recruit enzymes so that every nascent protein is ubiquitinated and thus is tagged for immediate proteolysis.

RISC may interfere with initiation of translation by interfering with 5' cap binding by ribosomes.

RISC may interfere with initiation of translation by preventing assembly of the ribosomal subunits.

RISC may remove the 3' poly(A) tail from mRNAs, hastening the degradation of these mRNAs.

RISC may interfere with the elongation stage of translation

RISC may completely stop transcription in the middle of an intron so that one of the splice sites is not transcribed, thus interfering with proper
codon in the mRNA.


Need all four answers for the question with one line explanation
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Answer 1

Answer:

When we talk about The risc complex it directly means that we are talking about Rna silencing

In Rna silecing

Let's take an example

Explanation:

Tobacco plant is affected by meliodegyne incognitia a type of worm that cause root knot disease

So to save tobacco plant rna silencing is used

in this process we first alter the gene which cause knot in roots and by making their pair we make a gene and add it to the gametes or embryo of tobacco plant

When the gene produces protein it is dsrna which is not supported by the cell structure (cell mediated mechanism) and this cutted by splicing enzyme

when the worm uses this protein to cause disease these cutted fragments attach to the ssrna and thus form dsrna again which is again cutted by splicing enzyme and thus ultimately the worm not able to live there